Head of the HIV-1 RNA Trafficking Laboratory, Lady Davis Institute
Member of the McGill University AIDS Centre
Professor, Department of Medicine; Associate Member of Microbiology & Immunology, McGill University
Dr. Andrew J. Mouland is head of the HIV-1 RNA Trafficking Laboratory and member of the McGill University AIDS Centre at the Lady Davis Institute (LDI), Associate Professor of Medicine and of Microbiology and Immunology at McGill University, and Adjunct Professor at the Université de Montréal. He is an internationally recognized scientist in the field of HIV-1 RNA and cell biology. Dr. Mouland has obtained career awards from the Canadian Institutes of Health Research (CIHR) as a New Investigator (2003-2008) and from the FRSQ as Chercheur-boursier senior (2008-2010). He is a member of several McGill University academic and executive committees, has chaired several committees at the LDI, and has served on advisory and grant review committees for the CIHR. He reviews grant requests for CIHR, NSERC, CANFAR, Manitoba Health Research Council HIV/AIDS and SARS-CoV streams, and reviews articles for journals such as the Journal of Cell Science, the Journal of Biological Chemistry, Nucleic Acids Research, Current HIV-1 Research, AIDS, Retrovirology, Virology, the Journal of General Virology, Biotechnology Progress, and has served as an advisor to Canadian publishing houses. He organized the American Society for Cell Biology conference on The Cell Biology of HIV-1 and Other Retroviruses held in Atlanta in July 2006.
Major Research Activities
Dr. Mouland runs a medium-sized lab with young and highly talented molecular virologists who study the molecular biology of human immunodeficiency virus type 1 (HIV-1), the causative agent of AIDS. Specifically, they have identified several key cellular proteins that determine the fate of HIV-1 RNA in the cell. These proteins mark HIV-1 RNAs and mediate trafficking of HIV-1 transcripts from the nucleus into the cytosol, then to sites of viral assembly and, finally, into the virus. Other proteins identified by members of the lab have important effects on the metabolism of HIV-1 RNAs in cells and the formation of HIV-1 specific ribonucleoprotein complexes. Using biochemical, genetic, in vitro, and in vivo techniques, combined with digital imaging and in vivo assays, including bioluminescence resonance energy transfer and live cell microscopy, they have provided pioneering insights into the roles of host cell proteins and machineries in these processes. Furthermore, they have undertaken a global approach to identify other relevant interactions between cellular and viral components that contribute to intracellular trafficking and the fate of viral proteins and RNAs. Their study will lead to a better understanding of fundamental cellular processes (e.g, RNA trafficking, endosomal trafficking, RNA translation and metabolism), as well as to provide for a more complete understanding of the cellular process that HIV-1 commandeers for efficient replication and generation of infectious virus particles. Of particular interest, and subjects for graduate and post-doctoral work, are: 1) HIV-1 evasion of host RNA quality control machineries; 2) HIV-1 evasion of host stress responses; 3) characterization of the Staufen1 HIV-1-dependent ribonucleoproteins (SHRNPs); and 4) medium- to high-throughput screening for host genes and machineries involved in the evasion RNA quality control and stress response machineries.
Fernando Valiente-Echeverría, Luca Melnychuk, Kishanda Vyboh, Lara Ajamian, Imed-Eddine Gallouzi, Nicole Bernard & Andrew J. Mouland. eEF2 and Ras-GAP SH3 domain-binding protein (G3BP1) modulate stress granule assembly during HIV-1 infection. 2014 Nature Communications. doi:10.1038/ncomms5819.
Levon Abrahamyan, Laurent Chatel-Chaix, Lara Ajamian, Miroslav P. Milev, Anne Monette, Jean-Francois Clément, Rujun Song, Martin Lehmann, Luc DesGroseillers, Michael Laughrea, Graciela Boccaccio and Andrew J. Mouland. Novel Class of Staufen1 Ribonucleoproteins Prevents Stress Granule Formation but Favours HIV-1 Genomic RNA Encapsidation. 2010. J. Cell. Sci. 123 (3): 369-383.
Anne Monette, Lara Ajamian, Marcelo Lopez-Lastra, Andrew J. Mouland. hnRNP A1 is retained in the cytoplasm in HIV-1 infected cells due to Nup62 degradation. 2009. J. Biol. Chem. 284(45): 31350-31362.
Martin Lehmann, Miroslav Milev, Levon Abrahamyan, X.-J. Yao, Nelly Pante and Andrew J. Mouland. Intracellular Transport of HIV-1 genomic RNA and Viral Production are Dependent on Dynein and Late Endosome Positioning. 2009. J. Biol Chem. 284 (21):14572-14585.