LDI Flow Cytometry Facility
Our mission is to provide
complete flow cytometry analysis and cell sorting services in terms of
experimental design, instrumentation, training, technical assistance and scientific
guidance.
LDI Flow Cytometry Facility Overview
The fee-for-service Lady Davis Institute Flow
Cytometry Facility provides researchers and clinicians with instrumentation,
scientific expertise and guidance. It consists of two flow cytometer analyzers:
a BD LSR Fortessa and a BD FACSCanto II) and a BSL 2 cell sorter (BD FACSAria
Fusion). It is also equipped with an automated cell counter. The CellDrop FL
cell counter (Denovix) allows accurate cell counting and cell size
determination. Several flow cytometry data analysis stations are also
available.
What is flow cytometry?
With an array of lasers, prisms, mirrors and
filters, flow cytometry instruments enable us to look at several physical and
fluorescent parameters of a single cell. These physical and fluorescent
parameters can be analyzed simultaneously, and since flow cytometers can
analyze hundreds of cells per second, large populations of cells can easily be
quantitatively characterized. It is this parameter multiplexing capability that
makes flow cytometry an extremely versatile and powerful tool for both
researchers and clinicians. In addition, fluorescence activated cell sorters
can physically separate these populations in different vessels or deposit
single cells into a multiwell plate for cloning.
Instrumentation