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LDI Flow Cytometry Facility
 

What is flow cytometry?

With an array of lasers, prisms, mirrors and filters, flow cytometry instruments enable us to look at several physical and fluorescent parameters of a single cell. These parameters can then be analyzed simultaneously. Since flow cytometers can analyze hundreds of cells per second, large populations of cells can easily be quantitatively characterized. It is this parameter multiplexing capability that makes flow cytometry an extremely versatile and powerful tool for both researchers and clinicians. In addition, fluorescence activated cell sorters can physically separate these populations in different vessels or deposit single cells into a multiwell plate for cloning.

LDI Flow Cytometry Facility Overview

The fee-for-service Lady Davis Institute Flow Cytometry Facility provides researchers and clinicians with instrumentation, scientific expertise and guidance. It consists of three flow cytometer analyzers: a BD LSR Fortessa and two FACSCaliburs, Beckton-Dickinson) and a cell sorter (FACSVantage SE, Beckman-Dickinson). It is also equipped with an automated cell counter. The Z2 Coulter Counter (Beckman Coulter) allows accurate cell counting and cell size determination. Two analysis computers and several flow cytometry data analysis software are also available. Please find a detailed description of the instruments, software and services below.
 
Equipment and Service

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