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Peptide Synthesis Laboratory

Our core lab at the MCETC specializes in various custom and contract services related to peptide science. Peptides are complex molecules and each peptide sequence is unique with regard to its chemical and physical properties.

Peptides are synthesized by the solid-phase method using Fmoc chemistry on an Omega 348 multiple peptide synthesizer from Advanced ChemTech. Synthesis is carried out at 50 µmole or 100 µmole scales.

On average, a synthesis scale of 50 µmoles will yield about 20-25 mg of a crude 10-residue peptide, or 40-50 mg of a 20-mer after cleavage from the resin.

The Peptide Synthesis Laboratory provides a complete spectrum of services that include preparation of the peptide-resin, cleavage, peptide modifications such as cyclization, biotinylation, N-acetylation, C-amidation etc.

Strict quality control in peptide synthesis is very important. To ensure the highest quality of synthetic product, an analytical-scale high pressure liquid chromatogram (HPLC) is provided with every peptide. The core lab will also provide MALDI-TOF or electro-spray mass-spectra.


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