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Introduction to Flow Cytometry 

Flow cytometry is a method for quantifying components or structural features of cells by optical means. It  measures individual cells as they pass in a single file through a laser beam.
 
The amount of light diffracted at no angle (Forward Light Scatter; FSC) and at a 90o angle (Side Scatter, SSC) to the laser is detected. FSC is related to size and SSC is related to granularity. In addition, cells can be labeled with fluorescent dyes and quantitated to characterize cell properties.
 
Using this information, it is possible to identify cells as well as distinguish cell types in a mixture.

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